Is this project an undergraduate, graduate, or faculty project?
Undergraduate
Project Type
group
Campus
Daytona Beach
Authors' Class Standing
Arden Rodriguez, Sophomore Meliah Martin , Sophomore Graeme Grainger, Senior Arineh Shahbazi, Sophomore
Lead Presenter's Name
Arden Rodriguez
Lead Presenter's College
DB College of Arts and Sciences
Faculty Mentor Name
Dr. Amber Paul
Abstract
Chronic and acute wounds remain a significant clinical challenge, particularly in austere and resource-limited environments where access to advanced care is restricted. Non-Thermal Plasma (NTP) has emerged as a promising, non-invasive modality with reported antimicrobial and regenerative properties; however, its mechanisms of action and effects on human skin tissue require further characterization. The scope of this project is to engineer and utilize a 3D human skin epithelial model to evaluate the effects of NTP on wound healing. We will first build a 3D epithelial tissue model that will simulate human skin, followed by modeling and evaluation of wound pathology and healing with NTP therapy. Assessment of cell viability, cell communication, and proliferation/cellular turnover will be determined as primary metrics for evaluation. The primary goal of this project is to confirm if NTP promotes wound healing. We hypothesize that NTP exposure will enhance wound healing by promoting cellular regeneration and maintain tissue integrity without inducing cytotoxic effects. Findings from this study will provide critical insight into the biological effects of NTP on human skin and support its development as a safe, portable, and non-invasive therapeutic strategy. Ultimately, this work seeks to advance innovative wound care solutions suitable for deployment in environments with limited medical infrastructure.
Did this research project receive funding support (Spark, SURF, Research Abroad, Student Internal Grants, Collaborative, Climbing, or Ignite Grants) from the Office of Undergraduate Research?
No
Included in
Biomedical Devices and Instrumentation Commons, Cell Biology Commons, Skin and Connective Tissue Diseases Commons
Assessing Non-Thermal Plasma in Cutaneous Regeneration
Chronic and acute wounds remain a significant clinical challenge, particularly in austere and resource-limited environments where access to advanced care is restricted. Non-Thermal Plasma (NTP) has emerged as a promising, non-invasive modality with reported antimicrobial and regenerative properties; however, its mechanisms of action and effects on human skin tissue require further characterization. The scope of this project is to engineer and utilize a 3D human skin epithelial model to evaluate the effects of NTP on wound healing. We will first build a 3D epithelial tissue model that will simulate human skin, followed by modeling and evaluation of wound pathology and healing with NTP therapy. Assessment of cell viability, cell communication, and proliferation/cellular turnover will be determined as primary metrics for evaluation. The primary goal of this project is to confirm if NTP promotes wound healing. We hypothesize that NTP exposure will enhance wound healing by promoting cellular regeneration and maintain tissue integrity without inducing cytotoxic effects. Findings from this study will provide critical insight into the biological effects of NTP on human skin and support its development as a safe, portable, and non-invasive therapeutic strategy. Ultimately, this work seeks to advance innovative wound care solutions suitable for deployment in environments with limited medical infrastructure.