Abstract Title

Association of Salivary Cortisol Levels with Introversive and Extroversive personalities

Is this project an undergraduate, graduate, or faculty project?

Undergraduate

group

Daytona Beach

Authors' Class Standing

Pablo Robles, Freshman Alexander Teigen, Freshman

Lead Presenter's Name

Alexander Teigen

Lead Presenter's College

DB College of Arts and Sciences

Faculty Mentor Name

Alba Chabez

Abstract

Secretion of cortisol is regulated by a hypothalamic hormone and a pituitary hormone. Cortisol is a stress hormone that is secreted in response to a physical or emotional stressor. Different factors influence cortisol levels, including physical activity, stress and personality. The present study examined associations between introversive and extroversive personalities and cortisol salivary levels in saliva samples collected from Embry-Riddle students. A scientific personality questionnaire was used to classify the subjects. Competitive ELISA uses cortisol standards and salivary samples to compete with cortisol conjugated to horseradish peroxidase to detect binding sites on a microtiter plate with coated anti-Cortisol antibodies. Bounded cortisol enzyme conjugates are measured by the reaction to the peroxidase to the substrate tetramethylbenzidine (TMB). Optical density is read on a plate reader, and the amount of cortisol in the sample is inversely proportional to the amount of enzyme conjugate detected from the plate well. The characterization of cortisol levels is classified by utilizing a standard curve line. Future studies will include screening of a larger student population size for a more statistically accurate screening.

Did this research project receive funding support (Spark, SURF, Research Abroad, Student Internal Grants, or Ignite Grants) from the Office of Undergraduate Research?

No

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Association of Salivary Cortisol Levels with Introversive and Extroversive personalities

Secretion of cortisol is regulated by a hypothalamic hormone and a pituitary hormone. Cortisol is a stress hormone that is secreted in response to a physical or emotional stressor. Different factors influence cortisol levels, including physical activity, stress and personality. The present study examined associations between introversive and extroversive personalities and cortisol salivary levels in saliva samples collected from Embry-Riddle students. A scientific personality questionnaire was used to classify the subjects. Competitive ELISA uses cortisol standards and salivary samples to compete with cortisol conjugated to horseradish peroxidase to detect binding sites on a microtiter plate with coated anti-Cortisol antibodies. Bounded cortisol enzyme conjugates are measured by the reaction to the peroxidase to the substrate tetramethylbenzidine (TMB). Optical density is read on a plate reader, and the amount of cortisol in the sample is inversely proportional to the amount of enzyme conjugate detected from the plate well. The characterization of cortisol levels is classified by utilizing a standard curve line. Future studies will include screening of a larger student population size for a more statistically accurate screening.