Abstract Title
Association of Salivary Cortisol Levels with Introversive and Extroversive personalities
Is this project an undergraduate, graduate, or faculty project?
Undergraduate
group
Daytona Beach
Authors' Class Standing
Pablo Robles, Freshman Alexander Teigen, Freshman
Lead Presenter's Name
Alexander Teigen
Lead Presenter's College
DB College of Arts and Sciences
Faculty Mentor Name
Alba Chabez
Abstract
Secretion of cortisol is regulated by a hypothalamic hormone and a pituitary hormone. Cortisol is a stress hormone that is secreted in response to a physical or emotional stressor. Different factors influence cortisol levels, including physical activity, stress and personality. The present study examined associations between introversive and extroversive personalities and cortisol salivary levels in saliva samples collected from Embry-Riddle students. A scientific personality questionnaire was used to classify the subjects. Competitive ELISA uses cortisol standards and salivary samples to compete with cortisol conjugated to horseradish peroxidase to detect binding sites on a microtiter plate with coated anti-Cortisol antibodies. Bounded cortisol enzyme conjugates are measured by the reaction to the peroxidase to the substrate tetramethylbenzidine (TMB). Optical density is read on a plate reader, and the amount of cortisol in the sample is inversely proportional to the amount of enzyme conjugate detected from the plate well. The characterization of cortisol levels is classified by utilizing a standard curve line. Future studies will include screening of a larger student population size for a more statistically accurate screening.
Did this research project receive funding support (Spark, SURF, Research Abroad, Student Internal Grants, or Ignite Grants) from the Office of Undergraduate Research?
No
Association of Salivary Cortisol Levels with Introversive and Extroversive personalities
Secretion of cortisol is regulated by a hypothalamic hormone and a pituitary hormone. Cortisol is a stress hormone that is secreted in response to a physical or emotional stressor. Different factors influence cortisol levels, including physical activity, stress and personality. The present study examined associations between introversive and extroversive personalities and cortisol salivary levels in saliva samples collected from Embry-Riddle students. A scientific personality questionnaire was used to classify the subjects. Competitive ELISA uses cortisol standards and salivary samples to compete with cortisol conjugated to horseradish peroxidase to detect binding sites on a microtiter plate with coated anti-Cortisol antibodies. Bounded cortisol enzyme conjugates are measured by the reaction to the peroxidase to the substrate tetramethylbenzidine (TMB). Optical density is read on a plate reader, and the amount of cortisol in the sample is inversely proportional to the amount of enzyme conjugate detected from the plate well. The characterization of cortisol levels is classified by utilizing a standard curve line. Future studies will include screening of a larger student population size for a more statistically accurate screening.