Is this project an undergraduate, graduate, or faculty project?
Undergraduate
Project Type
individual
Campus
Daytona Beach
Authors' Class Standing
Rahela Dolha, Sophomore
Lead Presenter's Name
Rahela Dolha
Lead Presenter's College
DB College of Engineering
Faculty Mentor Name
Foram Madiyar
Abstract
IL-6 is a cell-signaling protein pair (antibody-antigen) scientifically validated and recognized as a biomarker of inflammation. During the onset of inflammation, IL-6 antigens are released into the bloodstream to signal the production of other pro-inflammatory proteins. As IL-6 antigens circulate in the bloodstream, ~10% of the concentration is filtered and released through perspiration. This presents IL-6 as a very useful resource for the monitoring of inflammation, a very persistent and painful symptom of chronic illnesses. This project highlights the development and optimization of a point-of-care (POC) impedance biosensor. An interleukin-6 (IL-6) sensing two-electrode system is used as a tool for lightweight and rapid monitoring of inflammation. The two-electrode system is modified with the following layers on the working electrode (ordered bottom to top): gold nanoparticle-coated cellulose nanofibers, a polymer-protein crosslinker, and IL-6 antibodies. Theoretically, when perspired IL-6 antigens come into contact with this biosensor, the impedance of the system notably increases. This contact will be simulated through a saline solution of IL-6 antibodies dropped onto the channel of system. The impedance of the system is measured and recorded to determine the accuracy and sensitivity of the sensor. The biosensor will be developed as a POC biosensor due to the increase in accessibility that they provide. POC biosensors are ready-to-use, cost-effective, and portable sensors that can greatly benefit the underprivileged, rural or isolated communities, the elderly and disabled, and those in hazardous environments (oil rigs, space, military camps, submarines, planes, etc.). Individuals with limited or no access to medical care or testing centers due to financial, geographical, or physical limitations would be able to receive the necessary tests and continuous monitoring needed. By providing affordable, easy-to-use diagnostics, this POC impedance biosensor ensures rapid testing without the need for a medical professional, thereby providing equitable access to healthcare across all populations.
Did this research project receive funding support (Spark, SURF, Research Abroad, Student Internal Grants, Collaborative, Climbing, or Ignite Grants) from the Office of Undergraduate Research?
No
Development of a Portable, Low-Cost Sensor for the Detection of Inflammation
IL-6 is a cell-signaling protein pair (antibody-antigen) scientifically validated and recognized as a biomarker of inflammation. During the onset of inflammation, IL-6 antigens are released into the bloodstream to signal the production of other pro-inflammatory proteins. As IL-6 antigens circulate in the bloodstream, ~10% of the concentration is filtered and released through perspiration. This presents IL-6 as a very useful resource for the monitoring of inflammation, a very persistent and painful symptom of chronic illnesses. This project highlights the development and optimization of a point-of-care (POC) impedance biosensor. An interleukin-6 (IL-6) sensing two-electrode system is used as a tool for lightweight and rapid monitoring of inflammation. The two-electrode system is modified with the following layers on the working electrode (ordered bottom to top): gold nanoparticle-coated cellulose nanofibers, a polymer-protein crosslinker, and IL-6 antibodies. Theoretically, when perspired IL-6 antigens come into contact with this biosensor, the impedance of the system notably increases. This contact will be simulated through a saline solution of IL-6 antibodies dropped onto the channel of system. The impedance of the system is measured and recorded to determine the accuracy and sensitivity of the sensor. The biosensor will be developed as a POC biosensor due to the increase in accessibility that they provide. POC biosensors are ready-to-use, cost-effective, and portable sensors that can greatly benefit the underprivileged, rural or isolated communities, the elderly and disabled, and those in hazardous environments (oil rigs, space, military camps, submarines, planes, etc.). Individuals with limited or no access to medical care or testing centers due to financial, geographical, or physical limitations would be able to receive the necessary tests and continuous monitoring needed. By providing affordable, easy-to-use diagnostics, this POC impedance biosensor ensures rapid testing without the need for a medical professional, thereby providing equitable access to healthcare across all populations.