Presenter Information

John E. Love
Glenn M. CohenFollow

Location

Howard Johnson Plaza-Hotel, Columbia/ Enterprise Rooms

Start Date

24-4-1990 2:00 PM

End Date

24-4-1990 5:00 PM

Description

The basilar papilla is the hearing portion of the bird f s inner ear and serves as a model for human inner ear development. We used neuron-specific enolase (NSE) as a marker for the chick T s auditory nerve (statoacoustic ganglion) development. The chick hatches after an incubation period of 3 weeks. At stage 38 (12th embryonic day), the earliest stage examined in the present experiments, we observed faint NSE staining (immunoreactivity) in neurons of the statoacoustic ganglion. By stage 39- «13th embryonic day), stronger NSE immunoreacti vi ty appeared in most neuronal cell bodies (perikarya). By stage 46 (21st day [hatching]), neurons stained strongly for NSE. Thus, NSE levels (immunoreactivities) increase during development and correspond to neuronal differentiation.

Comments

Science Payloads

Session Chairman: Joe Alexander, NASA Assistant Associate Administrator for Space Science and Applications, NASA Headquarters

Session Organizer: Lee O’Fallon, NASA, Kennedy Space Center

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Apr 24th, 2:00 PM Apr 24th, 5:00 PM

Paper Session I-B - Developmental Appearance of Neuron-Specific Enolase in the Embryonic Chick's Basilar Papilla

Howard Johnson Plaza-Hotel, Columbia/ Enterprise Rooms

The basilar papilla is the hearing portion of the bird f s inner ear and serves as a model for human inner ear development. We used neuron-specific enolase (NSE) as a marker for the chick T s auditory nerve (statoacoustic ganglion) development. The chick hatches after an incubation period of 3 weeks. At stage 38 (12th embryonic day), the earliest stage examined in the present experiments, we observed faint NSE staining (immunoreactivity) in neurons of the statoacoustic ganglion. By stage 39- «13th embryonic day), stronger NSE immunoreacti vi ty appeared in most neuronal cell bodies (perikarya). By stage 46 (21st day [hatching]), neurons stained strongly for NSE. Thus, NSE levels (immunoreactivities) increase during development and correspond to neuronal differentiation.

 

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