Submitting Campus
Daytona Beach
Department
Human Factors and Behavioral Neurobiology
Document Type
Article
Publication/Presentation Date
10-20-2015
Abstract/Description
Chikungunya virus (CHIKV) is a mosquito-transmitted alphavirus that can cause fever and chronic arthritis in humans. CHIKV that is generated in mosquito or mammalian cells differs in glycosylation patterns of viral proteins, which may affect its replication and virulence. Herein, we compare replication, pathogenicity, and receptor binding of CHIKV generated in Vero cells (mammal) or C6/36 cells (mosquito) through a single passage. We demonstrate that mosquito cell derived CHIKV (CHIKVmos) has slower replication than mammalian cell derived CHIKV (CHIKVvero), when tested in both human and murine cell lines. Consistent with this, CHIKVmos infection in both cell lines produce less cytopathic effects and reduced antiviral responses. In addition, infection in mice show that CHIKVmos produces a lower level of viremia and less severe footpad swelling when compared with CHIKVvero. Interestingly, CHIKVmos has impaired ability to bind to glycosaminoglycan (GAG) receptors on mammalian cells. However, sequencing analysis shows that this impairment is not due to a mutation in the CHIKV E2 gene, which encodes for the viral receptor binding protein. Moreover, CHIKVmos progenies can regain GAG receptor binding capability and can replicate similarly to CHIKVvero after a single passage in mammalian cells. Furthermore, CHIKVvero and CHIKVmos no longer differ in replication when N-glycosylation of viral proteins was inhibited by growing these viruses in the presence of tunicamycin. Collectively, these results suggest that N-glycosylation of viral proteins within mosquito cells can result in loss of GAG receptor binding capability of CHIKV and reduction of its infectivity in mammalian cells.
Publication Title
PLOS Neglected Tropical Diseases
DOI
https://doi.org/10.1371/journal.pntd.0004139
Publisher
Public Library of Science
Grant or Award Name
Faculty startup fund (DE01363) from The University of Southern Mississippi
Scholarly Commons Citation
: Acharya D, Paul AM, Anderson JF, Huang F, Bai F (2015) Loss of Glycosaminoglycan Receptor Binding after Mosquito Cell Passage Reduces Chikungunya Virus Infectivity. PLoS Negl Trop Dis 9 (10): e0004139. doi:10.1371/journal.pntd.0004139
Included in
Behavioral Neurobiology Commons, Immunity Commons, Medical Immunology Commons, Neurology Commons, Virology Commons
Additional Information
Dr. Paul was not affiliated with Embry-Riddle Aeronautical University at the time this paper was published.