Submitting Campus
Daytona Beach
Department
Human Factors and Behavioral Neurobiology
Document Type
Article
Publication/Presentation Date
8-24-2016
Abstract/Description
Zika virus (ZIKV) is a globally emerging mosquito-transmitted flavivirus that can cause severe fetal abnormalities, including microcephaly. As such, highly sensitive, specific, and cost-effective diagnostic methods are urgently needed. Here, we report a novel electrogenerated chemiluminescence (ECL)-based immunoassay for ultrasensitive and specific detection of ZIKV in human biological fluids. We loaded polystyrene beads (PSB) with a large number of ECL labels and conjugated them with anti-ZIKV monoclonal antibodies to generate anti-ZIKV-PSBs. These anti-ZIKV-PSBs efficiently captured ZIKV in solution forming ZIKV-anti-ZIKV-PSB complexes, which were subjected to measurement of ECL intensity after further magnetic beads separation. Our results show that the anti-ZIKV-PSBs can capture as little as 1 PFU of ZIKV in 100 μl of saline, human plasma, or human urine. This platform has the potential for development as a cost-effective, rapid and ultrasensitive assay for the detection of ZIKV and possibly other viruses in clinical diagnosis, epidemiologic and vector surveillance, and laboratory research.
Publication Title
Scientific Reports
DOI
https://doi.org/10.1038/srep32227
Publisher
Nature Publishing Group
Scholarly Commons Citation
Paul, A. M., Acharya, D., Bastola, P., Le, L., Fernandez, E., Diamond, M. S., Miao, W., & Bai, F. (2016). An Ultrasensitive Electrogenerated Chemiluminescence-Based Immunoassay for Specific Detection of Zika Virus. Scientific Reports, 6(). https://doi.org/10.1038/srep32227
Included in
Behavioral Neurobiology Commons, Immunity Commons, Medical Immunology Commons, Neurology Commons, Neurosciences Commons, Virology Commons
Additional Information
Dr. Paul was not affiliated with Embry-Riddle Aeronautical University at the time this paper was published.